Luminescence Lifetime Imaging Microscopy by Confocal Pinhole Shifting (LLIM-CPS).

Luminescence Lifetime Imaging Microscopy by Confocal Pinhole Shifting (LLIM-CPS).

125 pages· 2007· ISBN 9780549326878
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LLIM-CPS was used to quantify oxygen-dependent changes in intensity and lifetime of Tris-4, 7 diphenyl 1, 10-phenanthroline ruthenium an oxygen sensing long lifetime luminophore. LLIM-CPS images of heart cells in the presence of the oxygen-sensing phosphorescent luminophore, PtTBP-AG2-PEG, visualized oxygen surrounding the respiring cells. Thus, in this dissertation I have demonstrated an adaptation of a LSCM to perform quantitative long lifetime luminescence imaging and presented a biological application of oxygen sensing with this technique.

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