Mapping QTL for Agronomic and Canning Quality Traits in Black Bean (Phaseolus Vulgaris L.).

Quantitative trait loci (QTL) analysis was used to identify QTL for agronomic performance and canning quality in a recombinant inbred line (RIL) population from the cross of the black bean cultivar 'Jaguar' and the breeding line 115M. A total of 96 RILs were evaluated for seven agronomic and four canning quality traits in replicated trials at one location over four years (2004-2007) in Michigan. SSR, TRAP, SRAP, and phenotypic markers were used to create a genetic map of the population consisting of 119 loci including a locus associated with resistance to a new race of bean rust isolated in Michigan. The map consisted of 15 linkage groups spanning 460cM (38%) of the bean genome. Composite interval mapping analysis identified a total of 20 QTL for 10 traits averaged across environments, while an additional 18 QTL were identified in one or more individual environments. QTL were identified on 10 linkage groups (LG). A major QTL for seed yield was identified on LG B10. A total of 7 QTL for yield, seed size, plant height, and canned bean texture showed positive alleles from 115M. Several QTL co-localized with regions identified in previous studies while others, particularly for canning quality, were unique. Rust resistance associated with 115M was mapped to LG B4 and flanked by two TRAP markers, both at an approximate distance of 3 cM. These results support the utility of TRAP markers to tag disease resistance loci and QTL and provide a valuable source of rust resistance for future black bean cultivars adapted to Michigan.