The Design and Development of the Cognate Site Identifier (CSI) Microarray to Determine the Comprehensive Sequence Specificity of Any DNA-binding Molecule

To date, we have examined the sequence specificity of numerous small molecules, transcription factors, and cooperative complexes. Most small molecules and transcription factors studied using the CSI microarray have yielded informative DNA-binding profiles, indicating that the binding preferences of almost any DNA-binding ligand can be obtained with this approach. Therefore, we believe the CSI microarray will become a widespread and invaluable technique in the study of any molecule that can interact with DNA.